MapSplice Project Homepage
Description
MapSplice is a novel algorithm for this problem, requiring only RNA-seq reads and a reference genome as input. Compared with previous methods, MapSplice does not rely on existing splicing databases, splice site recognition techniques or structural features commonly found at splice sites. Instead, it exhaustively aligns all splice junction reads to the genome with a gap length between a given minimum and maximum intron length. MapSplice infers splice sites from the alignment of splice junction reads and their abundance relative to nearby exonic reads. Putative splice sites with insufficient coverage by splice junction reads or inconsistent expression levels are filtered out.
Software
- MapSplice Manual
- Software Version Download Page
Project Discussion (collaborators only)