We recommend you first align your reads with MapSplice. Please refer to user guide for instructions on using MultiSplice.
Tips before using:
1. Please make sure the input annotation file is in GFF format;
2. Please make sure the read alignment file is in SAM format.
3. MultiSplice only works on single-end reads. In case of paired-end reads, please use MapPER to infer the transcript fragment first.
Source code and directory:
Latest version:MuliSplice v0.10 (5/5/2012)